THYROID

Instructions for use

The MESACUP-2 TEST anti-Tg is the quantitative enzyme-linked immunosorbent assay (ELISA) for the detection of anti-Thyrogloblin (Tg) antibodies in human serum.

Background:

Thyroglobulin (Tg) is grycoprotein produced in thyroid epitherial cell and located in thyroid follicle with molecular weight approximately 650,000. Autoantibodies against Tg molecule has been recognized as a typical organ specific autoanbody since Witebsky et al. in 1955, reported production of anti-Tg antibodies in the serum of Hashimoto’s disease like thyroiditis by allo-immunization of rabbit thyroid homogenate etc., and since Roitt et al. reported detection of anti-Tg antibodies in the serum of Hashimoto’s disease patient.Complement binding of anti-Tg antibodies are weak and pathologenic significance of direct cytotoxic activity like anti-microsomal antibodies are not clarified. But it is known to show high value in the blood of autoimmune thyroid diseases such as Hashimoto disease and Basedow’s disease. For this reason, detection of anti-Tg antibody in the serum is widely performed together with anti-microsomal antibodies for the diagnosis and indication of treatment of thyroid disease.
The MESACUP-2 TEST anti-Tg is reagent for detecting anti-Tg antibodies in the serum with ELISA method.

Principle:

The MESACUP-2 TEST anti-Tg measures anti-Tg antibodies present in the serum by ELISA. Standards and patient sera are added to microwell coated with Tg antigens, allowing anti-Tg antibodies to react with the immobilized antigen (Sample incubation). After wash to remove any unbound serum proteins, horseradish peroxidase conjugated anti human IgG polyclonal antibody (goat) is added and incubated (Conjugate incubation). Following another washing step, the peroxidase substrate is added and incubated for an additional period of time (Substrate incubation). Acid solution is then added to each well to terminate the enzyme reaction and to stabilize the color development. The assay can be quantified by measuring the reaction photometrically and plotting the results.

Size: 96 wells

Method: ELISA

Antigen: native

Availability: all regions

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