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Instructions for use

The MESACUP anti-p53 TEST is an Enzyme Linked Immuno-Sorbent assay (ELISA) detection kit for anti-p53 antibody in serum.

Background:

The autoantibodies to p53 protein（anti-p53 antibodies）are highly related to malignant diseases and rarely detected in healthy donors and patients with benign diseases. This immune response is correlated with the presence of a p53 gene mutation which leads to the accumulation of an ineffective p53 protein in the tumor cells.

Principle:

The MESACUP anti-p53 TEST measures anti-p53 antibodies in the serum by ELISA. Calibrators and patient serum are added to microwells coated with recombinant wild-type human p53 protein (to detect specific anti-p53 antibodies) and with control proteins (to detect non-specific interactions), allowing anti-p53 antibodies to react with the immobilized antigen (Sample incubation). After washing to remove any unbound serum proteins, horseradish peroxidase conjugated anti human IgG is added and incubated (Conjugate incubation). Following another washing step, the peroxidase substrate is added and incubated for an additional period of time (Substrate incubation). Acid solution is then added to each well to terminate the enzyme reaction and to stabilize the color development. The value in each sample can be obtained by comparing the OD of the sample to the OD of the anti-p53 standard.

Size: 48 wells

Method: ELISA

Antigen: recombinant p53 protein

Availability: all regions

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