CONNECTIVE TISSUE DISEASES

Instructions for use

The FLUORO nDNA TEST is intended for semi-quantitative detection of anti DNA antibodies in human serum.

Background:

Antinuclear antibodies can be found in the sera of patients with autoimmune diseases such as systemic lupus erythematosus (SLE). Especially, anti-native DNA antibodies (or anti-double stranded DNA antibodies) are highly specific for SLE. Therefore, detection of these antibodies is important in the diagnosis of SLE patients.
The mitochondrion (kinetoplast) of the Crithidia luciliae contains a large amount of ds-DNA but does not contain ss-DNA or histone. Therefore, the FLUORO nDNA TEST, which uses Crithidia luciliae as the substrate, is able to isolate and detect anti-nDNA antibodies with ease and accuracy.

Principle:

The FLUORO nDNA TEST detects anti nDNA antibodies by the indirect immuno-fluorescence method. Crithidia luciliae is used as the substrate, and fluorescein isothiocyanate (FITC) is used as fluorescent dye.

Size: 80 wells (4 wells x 20 slides)

Method: IIF

Substrate: Crithidia lucilia