Insulin receptor α-subunit ELISA TEST

For Research Use Only. Not for use in diagnostic procedures.

Instructions for use

The Insulin receptor α-subunit (IRα) ELISA TEST is an Enzyme Linked Immuno-Sorbent assay (ELISA) quantization kit for human IRα in serum and plasma.

Principle:

The IRα ELISA TEST measures human IRα in the serum and plasma by ELISA. Calibrators and patient serum/plasma are added to microwells coated with anti-human IRα (clone ), allowing IRα to react with the immobilized antibody (Sample incubation). After washing to remove any unbound IRα and serum proteins, biotin conjugated anti IRα (clone ) is added and incubated (Conjugate incubation). After washing away all unbound biotin labeled detection antibody, the peroxidase conjugated streptavidin (SA) is added and attaches to the biotin label on the bound detection antibody (Biotin-Avidin incubation). Following another washing step, the peroxidase substrate is added and incubated for an additional period of time (Substrate incubation). Acid solution is then added to each well to terminate the enzyme reaction and to stabilize the color development. The value in each sample can be obtained by comparing the OD of the sample to the OD of the IRα Calibrator.

Size: 96 wells

Method: ELISA

Availability: all regions