CONNECTIVE TISSUE DISEASES

Instructions for use

The FLUORO HEPANA TEST is intended for semi-quantitative detection of antinuclear antibodies in human serum.

Background:

Antinuclear antibodies (ANAs) are found in patients with autoimmune diseases such as systemic lupus erythematosus (SLE). Certain specific types of ANAs have been found to have important clinical correlations that make them useful for the diagnosis and treatment of patients with variety of rheumatic diseases. In the FLUORO HEPANA TEST, HEp-2 cells which are derived from human pharyngeal cancer, are used as the nuclear substrate. Since the nuclei of HEp-2 cells are large, the staining pattern can be observed in detail. Moreover, since cells in various stages of the cell cycle coexist, detection of anti centromere antibodies and anti PCNA (proliferating cell nuclear antigens) antibodies is also possible with the FLUORO HEPANA TEST.

Principle:

The FLUORO HEPANA TEST detects antinuclear antibodies by the indirect immunofluorescence. HEp-2 cells are used as nuclear substrates, and fluorescein isothiocyanate (FITC) is used as fluorescent dye.

Size: 160 wells (8 wells x 20 slides)

Method: IIF

Substrate: HEp-2 cells