MESACUP DNA-II TEST “ss”

IVD-In Vitro Diagnostic Use CE marked

Instructions for use

The MESACUP DNA-II TEST “ss” is the quantitative enzyme-linked immunosorbent assay (ELISA) for the detection of anti single-stranded (ss) DNA antibodies in human serum.

Background:

Antinuclear antibodies are found in autoimmune disease such as SLE. Among them, anti-DNA antibodies are characteristic for SLE, therefore, it is drawing wide attention as an agent to increase the accuracy of diagnosis. In recent studies, there are several reports from immunoglobulin class-wise measurement of anti-ssDNA antibodies that indicate its clinical availability. Particularly, Aotsuka et al. reported that anti-ssDNA antibodies in IgG class showed a significantly higher value in sera of SLE than in sera of others and correlated well with lupus urinary abnormality, and there were patients with SLE in active period that had anti-ssDNA antibodies in IgG class increased before turning to active period. So that the determination of both anti-ssDNA and anti-dsDNA antibodies in IgG class is of value for diagnosis and in making prognosis of this disease.

The MESACUP DNA-II TEST “ss” is for measuring anti ssDNA antibodies present in the serum by ELISA.

Principle:

The MESACUP DNA-II TEST “ss” measures anti-ssDNA antibodies present in the serum by ELISA. Standards and patient sera are added to microwell coated with ssDNA antigen, allowing anti-ssDNA antibodies to react with the immobilized antigen (Sample incubation). After wash to remove any unbound serum proteins, horseradish peroxidase conjugated anti human IgG monoclonal antibody (mouse) is added and incubated (Conjugate incubation). Following another washing step, the peroxidase substrate is added and incubated for an additional period of time (Substrate incubation). Acid solution is then added to each well to terminate the enzyme reaction and to stabilize the color development. The assay can be quantified by measuring the reaction photometrically and plotting the results.

Size: 96 wells

Method: ELISA

Antigen: bacteriophage λ DNA

Availability: all regions