CONNECTIVE TISSUE DISEASES

Instructions for use

The MESACUP ANA TEST is semi-quantitative enzyme-linked immunosorbent assay (ELISA) for the detection of disease specific anti-Nuclear antibodies in human serum.

Background:

Anti-dsDNA antibodies are highly specific for SLE and are detected at a high frequency in patients with active stage of disease. Several reports have demonstrated a correlation between disease activity and levels of anti-dsDNA antibodies in SLE. So the determination of anti-dsDNA antibodies is of value for early diagnosis and for making prognosis of SLE. The presence of antibodies to dsDNA in SLE has been included as one of the criteria for disease classification by the American College of Rheumatology. The ELISA method offers advantages over other assays (e.g. the PHA; passive hemagglutination, the Crithidia luciliae immunofluorescence assay and the Farr radioimmunoassay) in areas of sensitivity, specificity, objectivity and efficiency.

The MESACUP DNA-II TEST “ds” is for measuring anti dsDNA antibodies present in the serum with ELISA method.

Principle:

The MESACUP DNA-II TEST “ds” measures anti-dsDNA antibodies present in the serum by ELISA. Standards and patient sera are added to microwell coated with dsDNA antigen, allowing anti-dsDNA　antibodies to react with the immobilized antigen (Sample incubation). After wash to remove any unbound　serum proteins, horseradish peroxidase conjugated anti human IgG monoclonal antibody (mouse) is added and incubated (Conjugate incubation). Following another washing step, the peroxidase substrate is added and incubated for an additional period of time (Substrate incubation). Acid solution is then added to each well to terminate the enzyme reaction and to stabilize the color development. The assay can be quantified by measuring the reaction photometrically and plotting the results.

Size: 96 wells

Method: ELISA

Antigen: bacteriophage λ DNA