DIASTAT anti-CCP

IVD-In Vitro Diagnostic Use CE marked

Instructions for use

The DIASTAT anti-CCP is a semi-quantitative enzyme-linked immunosorbent assay (ELISA) for the detection of the IgG class of autoantibodies specific to cyclic citrullinated peptide (CCP) in human serum or plasma.

Background:

Rheumatoid arthritis (RA) is a common, systemic autoimmune disease affecting 0.5-1.0% of the adult population. RA is characterised by chronic inflammation of the synovium which can lead to progressive joint destruction and in many cases lead to disability and reduction of quality of life. It is generally accepted that early intervention is virtal in preventing irreversible joint damage and it is therefore important to diagnose RA as early in the disease course as possible.
For several years, it has been recognized that antibodies to anti-perinuclear factor (APF) and keratin (AKA) are highly specific for RA. Recent evidence has shown that all these antibodies are directed to citrulline containing epitopes. In 1988, Schellekens and colleagues reported that autoantibodies reactive with linear synthetic peptides containing citrulline were highly specific for RA in an ELISA based assay. Subsequent studies demonstrated that cyclic cariants of these linear peptides, termed cyclic citrullinated peptides (CCP) were as specific for RA but with a higher sensitivity than the linear peptides.
Anti-CCP antibodies have been found to be present very early in the disease, often with the absence of clinical symptoms and many reports indicate that elevated levels of anti-CCP antibodies can predict the development of erosive disease. These findings suggest an important role for cyclic citrullinated peptides in the diagnosis of RA at an early stage of the disease course.
The DIASTAT anti-CCP is an ELISA based on the detection of autoantibodies in human serum and plasma towards a synthetic cyclic peptide containing modified arginine residues (CCP2 peptides). The test provides an additional tool in the diagnosis of patients with RA.

Size: 96 wells

Method: ELISA

Antigen: a synthetic cyclic peptide containing modified arginine residues (CCP2 peptides)

Availability: Limited distribution to some countries in Asia