MESACUP BP180 TEST

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Instructions for use

The MESACUP BP180 TEST is a semi-quantitative enzyme-linked immunosorbent assay (ELISA) for the detection of anti BP180 antibodies in human serum.

Background:

Bullous pemphigoid (BP) is chronic itchy blistering disorder found mainly in aged person, characterized by frequent occurring of tense blister and erythema. IgG antibasement membrane zone (BMZ) antibodies are found in the serum of patients, and linear IgG or C3 sediment is found on the basement membrane zone of the lesion. Target antigens of the autoantibodies in BP patient serum are BP230 and BP180, also called BPAG1 and BPAG2. Molecular weight of these antigens is 230 kD and 180 kD respectively. BP180 is thought to be the direct target of the autoantibody because of its location, and the autoantibodies against BP230 are thought to be secondarily produced. The antibodies against BP180 are thought to be pathogenic, because the rabbit antibody against mouse NC16a region of BP180 forms blister similar to BP when injected to neonatal mice1). The main epitope of BP180 is located in the region close to cell membrane called NC16a and most of patient serum react with the recombinant NC16a protein2).

The MESACUP BP180 TEST is for measuring anti BP180 autoantibodies in patient serum specifically.

Principle:

The MESACUP BP180 TEST measures anti-BP180 antibodies present in the serum by ELISA. Calibrators and patient sera are added to microwell coated with BP180 antigen, allowing anti-BP180 antibodies to react with the immobilized antigen (Sample incubation). After washing to remove any unbound serum proteins, horseradish peroxidase conjugated anti-human IgG antibody is added and incubated (Conjugate incubation). Following another washing step, the peroxidase substrate is added and incubated for an additional period of time (Substrate incubation). Acid solution is then added to each well to terminate the enzyme reaction and to stabilize the color development. The assay can be quantified by measuring the reaction photometrically.

Size: 48 wells

Method: ELISA

Antigen: recombinant purified BP 180NC16a

Availability: all regions