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Fluorescence patterns of cytoplasmic autoantigens

Autoantibodies recognizing mitotic spindle
apparatus related antigens


The main component protein of the microtubles of the spindles is tubulin, and the microtubules bind to motor proteins like kinesin and dynein. Anti-spindle apparatus antibodies like NuMA antibodies possibly recognize the component proteins and the binding proteins of the microtubules.


Anti-spindle apparatus antibody


photo 55

Pattern The spindle fibers between the poles are stained in mitotic cells.
Antigen Tubulin
Clinical significance The presence of anti-tubulin antibodies were reported to occur in patients with parasitic infections and those with Guillain-Barre syndrome.
References 63, 64

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Anti-nuclear mitotic apparatus (NuMA)-1 antibody


photo 56

Pattern The spindle poles and the spindle fibers around them are stained in mitotic cells. Nuclear speckled staining can be seen in interphase cells.
Antigen 210kD protein
Other analytical methods WB
Clinical significance These antibodies occur in patients with autoimmune diseases like SS.
Reference 65

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Anti-nuclear mitotic apparatus (NuMA)-2 antibody


photo 57

Pattern The spindle poles and the spindle fibers are stained in mitotic cells. At anaphase/telophase, the staining is hifted to the intercellular bridge and the midbody. The nucleus and cytoplasm show no staining in the interphase cells.
Antigen kinesin-like protein HsEg5(130kD)
Other analytical methods Immunoprecipitation
Clinical significance These antibodies occur in patients with autoimmune diseases like SLE.
References 65, 66

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Anti-centriole antibody


photo 58


Pattern The poles of the mitotic spindle are stained in the mitotic cells and one or two dots are stained in the cytoplasm of the interphase cells.
Antigen 48kD heat shock proteins (enolase)
Clinical significance The antibodies occur infrequently in patients with Raynaud's syndrome and SSc.
References 67, 68

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SLE: systemic lupus erythematosus, SSc: systemic sclerosis, MCTD: mixed connective tissue disease, SS: Sjögren's syndrome, PM/DM: polymyositis/dermatomyositis, RA: rheumatoid arthritis, PBC: primary biliary cirrhosis, AIH: autoimmune hepatitis


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