Biochemical approach to identify disease pathways and functionally related genes
Tools for analysis of posttranscriptionally regulated and coordinated genes
Useful tools to study disease genes and novel mechanisms of drug action
Biochemical tools to improve microRNA target analysis
Post-transcriptional regulation of gene expression is a ribonucleoprotein (RNP)-driven process, which involves RNA binding proteins (RBPs) and noncoding RNAs that regulate splicing, nuclear export, subcellular localization, mRNA stability and translation. mRNAs that encode functionally related proteins are coordinately regulated during cellular processes such as proliferation, differentiation or drug treatment.
For example, mRNAs encoding proteins that function in a particular cell process or pathway can be found within a unique mRNP complex, which consists of mRNA and RBP.
By using anti-RBP antibodies, mRNAs which coexist with a certain RBP can be isolated and be analyzed.
RIP-Chip
RIP-chip is a technology used to identify functionally related RNAs and mRNA associated with RNA binding proteins (RBPs). RBPs are significant factors which lead to gene expression via the regulation of multiple mRNAs. This technology helps identify and understand functionally related genes in diseases. RIP-chip is a powerful and accurate tool that probes the mechanisms of post-transcriptional gene regulation.
RIP-chip works on the same principle as the widely used ChIP-chip.
It immunoprecipitates the ribonucleoprotein (RNP) from the cell extracts using an antibody raised against the RBP of interest. This simple procedure is then followed by microarray analysis. While microarrays determine the sequences of the RNA targets by hybridization, direct sequencing approaches (RIP-seq) can also be used to reveal RNA targets of RBPs.
RIP-chip or-seq data provide insights into new cellular pathway components leading to potential therapeutic targets and can also provide information regarding the effects of drugs on post-transcriptional processes. This technology can be applied to essentially any cellular system or animal model.
MBL provides RIP-Certified Antibodies that have been validated to work for this unique technology, as well as RIP-Assay Kit for yielding optimal results with these procedures.
＊RIP-Chip technology is patented by Ribonomics, Inc. (USP# 6,635,422、USP# 7,504,210 ).
MBL manufactures and distributes products under license from Ribonomics, Inc.
Products data sheets
◎ RIP Assay Kit (PDF:
508KB)
◎ RIP-Certified Antibody
| Product name | code no. | volume | data sheet |
|---|---|---|---|
| anti-EIF4E | RN001P | 200 uL | |
| anti-EIF4G1 | RN002P | 200 uL | |
| anti-EIF4G2 | RN003P | 200 uL | |
| anti-ELAVL1/HuR | RN004P | 200 uL | |
| anti-ELAVL2/HuB | RN005P | 200 uL | |
| anti-ELAVL3/HuC | RN006P | 200 uL | |
| anti-IGF2BP1/IMP1 | RN007P | 200 uL | |
| anti-IGF2BP2/IMP2 | RN008P | 200 uL | |
| anti-IGF2BP3/IMP3 | RN009P | 200 uL | |
| anti-MSI1/Musashi1 | RN010P | 200 uL | |
| anti-PTBP1 | RN011P | 200 uL | |
| anti-STAU1 | RN012P | 200 uL | |
| anti-STAU2 | RN013P | 200 uL | |
| anti-TIA1 | RN014P | 200 uL | |
| anti-YBX1 | RN015P | 200 uL | |
